Production of a full length Tat protein in E. coli and its purification

FEBS Letters

Lemay, Piere¹  de Nuova Perez, Lidia¹  Armengaud, Jean¹  Masson, Jean-Michel¹ 

[1]Institut National des Sciences Appliquées, Centre de Transfert en Biotechnologie et Microbiologie, Laboratoire de génie Biochimique, UA 544 du CNRS, Toulouse, France

关键词: HIV;    Tat;    Regulatory protein;    Gene expression;    Purification;   

DOI  :  10.1016/0014-5793(91)80467-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full length tat gene was constructed by a combination of a polymerase chain reaction (PCR) for the first exon and chemical synthesis for the second exon. This gene was expressed in E. Coli under the control of the strongly regulated araB promoter, either directly or fused to a secretion signal encoding sequence. We the defined a rapid, three-step procedure for the purification of the Tat protein

【 授权许可】

Unknown