The anionic conjugates of bilirubin and bile acids stimulate ATP hydrolysis by S‐(dinitrophenyl)glutathione ATPase of human erythrocyte

FEBS Letters

Singhal, Sharad S.¹  Lester, Roger²  Ahmad, Hassan¹  Awasthi, Yogesh C.¹  Sharma, Rajendra¹  Gupta, Sanjiv¹  Radominska, Anna²  Zimniak, Piotr² 

[1] Department of Human Biological Chemistry and Genetics, The University of Texas Medical Branch, Galveston, TX 77550, USA;Division of Gastroenterology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA

关键词: Glutathione-conjugates;    Dinitrophenylglutathione ATPase;    Bilirubin-conjugates;    Bile acid-conjugates;    Human liver;    Human erythrocytes;   

DOI  :  10.1016/0014-5793(91)80405-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

These studies demonstrate that bilirubin-ditaurate (an analog of bilirubin-diglucuronide), lithocholic acid 3-O-sulfate, and lithocholic acid 3-O-glucuronide, which are believed to be transported from liver into bile through an active transport process stimulate ATP hydrolysis by purified dinitrophenylglutathione ATPase of human erythrocytes. The K _m and V _ma? values of the enzyme for these substrates are similar to those for dinitrophenylglutathione indicating the transport mechanisms for bilirubin conjugates, and anionic bile acid-conjugates from hepatocytes to bile and transport of GSH-conjugates from erythrocytes may be mediated by similar mechanisms.

【 授权许可】

Unknown