Modification of the stability of phospholipase A2 by charge engineering

FEBS Letters

Perry, Barry¹  Warwicker, Jim¹  Bhat, K.Mahalingeshwara¹  Collins, Margaret E.¹  Goodenough, Peter W.¹  Pickersgill, Richard W.¹  Sumner, Ian G.¹ 

[1] Protein Engineering Department, AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading, RG29AT, UK

关键词: Protein engineering of phospholipase A2;    Charge engineering;    Protein stability;   

DOI  :  10.1016/0014-5793(91)80397-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Electrostatic interactions play an important role in stabilizing the folded conformation of globular proteins. Here we predict the change in stability of charge engineered mutants, construct these mutants and compare the predicted change in stability with that observed. The change in stability was correctly predicted for two of the three mutants and the factors responsible for the discrepancy between observation and prediction for the third mutant are discussed.

【 授权许可】

Unknown