Cloning of a glycine receptor subtype expressed in rat brain and spinal cord during a specific period of neuronal development

FEBS Letters

Hishinuma, Fumio¹  Akagi, Hiroyuki²  Hirai, Keiko¹ 

[1] Department of Molecular Biology, Mitsubishi Kasei Institute of Life Sciences, Tokyo 194, Japan;Department of Pharmacology, Gunma University School of Medicine, Gunma 371 Japan

关键词: Glycine receptor;    Chloride channel;    Messenger RNA;    cDNA cloning;    Xenopus oocytes;    Heterogeneity;    Northern blot;    Neuronal development;    NGlyR;    neonatal glycine receptor;    AGlyR;    adult glycine receptor;    E Cl;    equilibrium potential for chloride ion;    IC50;    concentration giving 50% inhibition;   

DOI  :  10.1016/0014-5793(91)80383-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Complementary (c) DNAs encoding a glycine receptor (GlyR) isomer were cloned from libraries constructed in λZAPII with poly (A)⁺ RNA of neonatal rat spinal cord. Northern blot analysis revealed that RNA hybridized to the cloned cDNA is detectable only for a period of late embryonic/ early postnatal stage of the spinal cord. Moreover, other central nervous tissues, such as hippocampus and cerebral cortex, in the infant rats are also rich in this message. The ‘neonatal (N) GlyR’ has 71% homology to that of another GlyR isoform in which adult rad cord is rich (AGlyR) Injection of a single RNA transcribed from the NGlyr-cDNA into Xenopus oocyte induced functional formation of glycine-gated Cl⁻ channels, however, its pharmacological property differed from that of AGlyR.

【 授权许可】

Unknown