What is the true ATPase activity of contracting myofibrils?

FEBS Letters

Travers, Franck¹  Barman, Tom¹  Houadjeto, Maurice¹ 

[1] INSERM U128, CNRS, BP 5051, 34033 Cedex 1 Montpellier, France

关键词: Myofibril;    Contraction;    Actomyosin;    ATPase activity;    S1;    myosin subfragment 1;   

DOI  :  10.1016/0014-5793(91)80369-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ATPase activity of contracting myofibrils was obtained in a solvent of ionic contents that mimic in vivo conditions: pH 7.4 and 0.1 M potassium acetate. Contracting myofibrils are fleeting structures and their ATPase activity is of short duration: even at 4°C it was over after a reaction time of 1 s and rapid reaction equipment was needed for its study. The ATPase (1.1 s⁻¹) was very similar to that of crosslinked actoS1 (1 s⁻¹). It was 100 × that of relaxed myofibrils.

【 授权许可】

Unknown