Rapid aggregation and tight junction formation in single cell suspensions of tumor cells after very low dose trypsin treatment

FEBS Letters

Kemmner, W.¹  Zaar, K.² 

[1] Institut für Biochemie II, Universität Heidelberg, Im Neuenheimer Feld 328, Heidelberg, Germany;Institut für Anatomie und Zellbiologie II, Universität Heidelberg, Im Neuenheimer Feld 307, W-6900 Heidelberg, Germany

关键词: Aggregation;    Tight junction;    Colon carcinoma cell;    Trypsin;   

DOI  :  10.1016/0014-5793(91)80354-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possible participation of tight junction formation in intercellular adhesion of tumor cells was studied in single cell suspensions of HT29 adenocarcinoma cells. Very low dose trypsin treatment (0.15 μg/ml, 30 min, 37°C) induced rapid intercellular adhesion of suspended HT29 colon carcinoma cells. Intercellular adhesion was independent of the presence of divalent cations. Electron microscopy of freeze-fractured membrane fragments of trypsin-treated HT29 cells revealed a dramatic increase of typical tight junction structures during aggregation.

【 授权许可】

Unknown