Studies of the small heat shock proteins of Caenorhabditis elegans using anti‐peptide antibodies

FEBS Letters

Clark-Lewis, Ian¹  Candido, E. Peter M.¹  Hockertz, Michael K.¹ 

[1] Department of Biochemistry University of British Columbia, Vancouver V6T IW5, Canada

关键词: Heat schock protein;    Caenorhabditis elegans;    α-Crystallin;    Aggregation;   

DOI  :  10.1016/0014-5793(91)80335-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peptides corresponding to selected regions of the 16 kDa small heat shock proteins (hsps) of the nematode C. elegans were synthesized and used to elicit polyclonal antibodies. It was found that these antibodies reacted predominantly with either the 16 kDa or the 18 kDa proteins, suggesting a close structural similarity between these hsps. Western blots of two-dimensional gels revealed extensive heterogeneity in these proteins, probably resulting from post-synthetic modifications. The native structures or both size classes or hsps were found to consist of large complexes of 4–5 × 10¹ Da.

【 授权许可】

Unknown