Electron microscopic and biochemical evidence that proline‐β‐naphthylamidase is composed of three identical subunits

FEBS Letters

Takahashi, Kenji²  Nishigai, Masaaki²  Ikai, Atsushi¹  Takahashi, Takayuki² 

[1] Laboratory of Biodynamics, Tokyo Institute of Technology, Nagatsuta, Midorik?, Yokohama 227, Japan;Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Proline-β-naphthylamidase;    Electron micrograph;    Trimer protein;    Subunit dissociation;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(91)80316-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Electron microscopy of pig intestinal proline-β-naphthyltamidase revealed that the enzyme is composed of 3 subunits, which are assembled in a trifoliolate shape, At pH 4.5 and 4°C, the enzyme dissociates reversibly into active subunits in 4h. Dissociation also occurs at higher pHs when the enyzme concentration is very low. The activity per mg protein of the native, trimeric enzyme is about 2.5-fold higher than that of the dissociated enzyme.

【 授权许可】

Unknown