Eukaryotic DNA primase abortive synthesis of oligoadenylates

FEBS Letters

Lavrik, O.I.¹  Podust, V.N.¹  Manakova, E.N.¹  Vladimirova, O.V.¹ 

[1] Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR

关键词: DNA primase;    DNA polymerase α;    Primer length;    PAGE;    polyacrylamide gel electrophoresis;    CT (HP) polα-primase;    calf thymus (human placenta) DNA polymerase α-primase;    HP primase;    human placenta DNA primase;    XC;    xylenecyanol;    BB;    Bromophenol blue;   

DOI  :  10.1016/0014-5793(91)80312-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calf thymus DNA polymerase α-primase, human placenta DNA polymerase α-primase and human placenta DNA primase synthesized oligoriboadenylates of a preferred length of 2–10 nucleotides and multimeric oligoribonucleotides of a modal length of about 10 monomers on a poly(dT) template. The dimer and trimer were the prevalent products of the polymerization reaction. However, only the oligonucleotides from heptamers to decamers were elongated efficiently by DNA polymerase α.

【 授权许可】

Unknown