Interferon‐γ binds to heparan sulfate by a cluster of amino acids located in the C‐terminal part of the molecule

FEBS Letters

Grimaud, Jean-Alexis¹  Lortat-Jacob, Hugues¹ 

[1] Laboratoire de Pathologie Cellulaire, CNRS URA 602, Institut Pasteur, Avenue Tony Garnier, 69007 Lyon, France

关键词: Interferon-γ;    Heparan sulfate;    Heparin;    Binding site;    Molecular stability;    IFN;    interferon;    Mab;    monoclonal antibody;    HS;    heparan sulfate;    HP;    heparin;   

DOI  :  10.1016/0014-5793(91)80225-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using three different approaches (domain mapping with monoclonal antibodies, limited enzymatic digestion and competition with synthetic peptides), we demonstrated that a cluster of basic amino acids on interferon-γ is involved in its binding to heparan sulfate. This cluster (Lys¹³¹=Arg¹²¹) is localized in the C-terminal part of IFN-γ. Once bound to heparan sulfate, IFN-γ is protected against protease attack.

【 授权许可】

Unknown