Epitope mapping of human thyroglobulin reveals a central immunodominant region

FEBS Letters

Henry, Mireille¹  Malthièry, Yves¹  Zanelli, Eric¹ 

[1] Laboratoire de Biochimie Médicale, INSERM U38, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France

关键词: Expression vector;    Fusion protein;    Immunoscreening;    Thyroglobulin;    Three-dimensional structure;   

DOI  :  10.1016/0014-5793(91)80146-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Thyroglobulin is the thyroid hormone precursor and the major antigen frequently involved in autoimmune diseases. The primary structure of human thyroglobulin is known but the spatial structure remains largely undetermined. By using fusion protein produced in prokaryotic system we have characterized seven short immunoreactive properties carrying at least one epitope. None of them includes hormonogenic sites, but five are concentrated in the central part of the monomeric molecule, which thus emerges as the major immunogenic region of this protein.

【 授权许可】

Unknown