A conformational switch involving the 915 region of Escherichia coli 16 S ribosomal RNA

FEBS Letters

Leclerc, Daniel¹  Brakier-Gingras, Léa¹ 

[1] Departement de Biochimie, Université de Montréal, Québec H3C 3J7, Canada

关键词: 16 S ribosomal RNA;    Streptomycin;    Translational accuracy;    Conformational switch;   

DOI  :  10.1016/0014-5793(91)80141-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A novel alternative conformation, which involves an interaction between the 5′ terminal and 915 regions (E. coli numbering), is proposed after a screening of compiled sequences of small subunit ribosomal RNAs. This conformation contains a pseudoknot helix between residues 12–16 and 911–915, and its formation requires the partial melting of the 5′ terminal helix and the disruption of the 17–19/916–918 pseudoknot helix of the classical 16 S rRNA secondary structure. The alternate pseudoknot helix is proximal to the binding site of streptomycin and various mutations in rRNA which confer resistance to streptomycin have been located in each strund of the proposed helix. It is suggested that the presence of streptomycin favours the shift towards the alternate conformation, thereby stabilizing drug binding. Mutations which destabilize the novel pseudoknot helix would restrict the response to streptomycin.

【 授权许可】

Unknown