Differential effect of extracellular calcium on the Na+ ‐K+ pump activity in intact polymorphonuclear leucocytes and erythrocytes

FEBS Letters

Johansen, Torben¹  Knudsen, Torben¹  Petersen, Reno H.¹ 

[1] Department of Pharmacology, Institute of Medical Biology, Odense University, J.B. Winslows Vej 19, DK-5000 Odense C, Denmark

关键词: Na+ -K+ pump;    Na+;    K+ -ATPase;    Calcium sodium;    Potassium;    Leucocyte;    Erythrocyte;   

DOI  :  10.1016/0014-5793(91)80272-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of extracellular calcium on the Na⁺ -K⁺ pump activity in human polymorphonuclear leucocytes and erythrocytes was studied and compared with the activity in mixed peritoneal leucocytes from rats. While there was maximal decrease in the pump activity (25–30%) of leucocytes from both rat and human by calcium 0.6 mM, a concentration of 0.1 mM caused a substantial decrease indicating a high sensitivity for extracellular calcium. In contrast, calcium had no effect on the pump activity in crythrocytes. The effect of calcium on the pump activity in leucocytes may be due to regulation of the influx of sodium across the plasma membrane, since in human leucocytes calcium had no effect on the pump activity if the cells were loaded with sodium.

【 授权许可】

Unknown