Induction of morphological change by tyrosine kinase inhibitors in Rous sarcoma virus‐transformed rat kidney cells

FEBS Letters

Sekizawa, Ryuichi¹  Tanaka, Karo¹  Abe, Satoko¹  Hori, Takashi¹  Imoto, Masaya¹  Umezawa, Kazuo¹ 

[1] Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohoma 223, Japan

关键词: Tyrosine kinase;    Erbstatin;    Sre;    Cytoskeleton;    Morphology;    Fibronectin;   

DOI  :  10.1016/0014-5793(91)80267-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Erbstatin and methyl 2,5-dihydroxycinnamate, related tyrosine kinase inhibitors, induced a morphological change in temperature-sensitive Rous sarcoma virus-transformed rat kidney (RSV¹⁵·NRK) that brought the cells close to the morphology of their normal counterpart. Erbstatin did not change the morphology of normal or Kirsten sarcoma virus-transformed rat kidney cells. Erbstatin also inhibited morphological transformation of RSV¹⁵·NRK cells induced by a shifting in temperature. Actin stress fibres were observed only in normal cells and not in transformed cells. Erbstatin induced stress fibre organization in transformed cells. Erbstatin and methyl 2.5-dihydroxycinnamate increased fibronectin gene expression in RSV-transformed cells. Thus, tyrosine kinase inhibitors induced normal phenotypes specifically in v-sre-expressing cells.

【 授权许可】

Unknown