Inositol 1,4,5‐trisphosphate activity in membranes isolated from amphibian skeletal muscle

FEBS Letters

Hidalgo, Cecilia¹  Carrasco, M.Angélica¹  Sánchez, Ximena¹  Vergara, Julio¹ 

[1] Centro de Estudios Cientificos de Santiago, Casilla 16443, Santiago 9, Chile and Departamento de Fisiologia y Biofisica, Facultad de Medicina, Universidad de Chile, Casilla 70055, Santiago, Chile

关键词: Excitation/contraction coupling;    Inositol phosphate;    Sarcoplasmic reticulum;    Transverse tubule;   

DOI  :  10.1016/0014-5793(91)80250-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hydrolysis of [³H]inositol 1,4,5-trisphosphate by a soluble fraction and by isolated transverse tubule and sarcoplasmic reticulum membranes from frog skeletal muscle was studied. Transverse tubule membranes displayed rates of hydrolysis several-fold higher than those of sarcoplasmic reticulum and soluble fraction; K _m and V _max were 25.2 μM and 44.1 nmol/mg/min, respectively. Transverse tubule membranes sequentially hydrolyzed inositol trisphosphate to inositol bisphosphate, inositol 1-phosphate and inositol. Indicating that these membranes have inositol bis- and monophosphatases in addition to inositol trisphosphatase.

【 授权许可】

Unknown