An upward shift of intracellular pH rather than the final absolute pH value is critical for controlling gap junction permeability in tumor promoter‐treated cells

FEBS Letters

Budunova, Irina¹  Mittelman, Leonid A.²  Margolis, Leonid B.² 

[1] All-Union Oncological Center of the USSR, Moscow, USSR;A.N. Belozersky Laboratory of Molekular Biology and Bioorganic Chemistry, Moscow State University, Moscow 119899, USSR

关键词: Cell—cell communication;    Intracellular pH;    BCECF;    bis(carboxyethyl)-5(6)caboxyfluorescein;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    PKC;    protein kinase C;   

DOI  :  10.1016/0014-5793(91)80248-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tumor promoter TPA is found to inhibit gap junction permeability in monolayer cultures of hamster fibroblasts. This effect is associated with an increase in intracellular pH. Here we show that neither an increase in pH₁ alone nor TPA treatment under conditions preventing pH₁-shift affect gap junction permeability. It is not the level of pH₁ reached, but rather the pH₁-shift itself that is essential for the inhibition of gap junction permeability in the presence of TPA.

【 授权许可】

Unknown