FEBS Letters | |
Effects of the general anaesthetic Propofol on the Ca2+‐induced permeabilization of rat liver mitochondria | |
Eriksson, Ove1  | |
[1] Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10, SF-00170 Helsinki 17, Finland | |
关键词: Antioxidant; Calcium; Mitochondria; Nonspecific permeability; Propofol; Swelling; BHT; butylated hydroxytoluene; tBHP; t-butylhydroperoxide; DMNTA; 2; 2-dimethylnitrilotriacetase; FCCP; carbonyl cyanide-p-trifluoromethoxyphenylhydrazone; PLA2; phosphatidyl 2-acylhydrolase (EC 3.1.1.4); Pi; inorganic phosphate; Propofol; 2; 6-di-isopropylphenol; RLM; rat liver mitochondria; ΔΨ; mitochondrial membrane potential; | |
DOI : 10.1016/0014-5793(91)80246-Y | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The molecular mechanisms of the Ca2+-induced permeabilization of rat liver mitochondria was evaluated by studying a new effect of the commonly used general anaesthetic Profol(2,6-diisopropylphenol). The compound was found to induce an apparent uptake of Ca2+ at steady-state in the Ca2+-distributed between the medium and the mitochondria, and to inhibit swelling and release of accumulated Ca2+ induced by inorganic phosphate, t-butyl hydroperoxide, diamide or FCCP plus Ruthenium red. The compound did not stimulate the activity of the Ca2+-uniporter and it is concluded that the effects seen are due to the inhibition of the Ca2+-dependent, unspecific permeability increase. The results suggest two mechanisms whereby Propofol stabilizes the mitochondrial membrane in the presence of Ca2+: (i) by interaction with the putative pore, thus causing its closure; and (ii) by scavenging of free radicals thus inhibiting its opening during oxidative stress.
【 授权许可】
Unknown
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