Effects of the general anaesthetic Propofol on the Ca2+‐induced permeabilization of rat liver mitochondria

FEBS Letters

Eriksson, Ove¹ 

[1] Department of Medical Chemistry, University of Helsinki, Siltavuorenpenger 10, SF-00170 Helsinki 17, Finland

关键词: Antioxidant;    Calcium;    Mitochondria;    Nonspecific permeability;    Propofol;    Swelling;    BHT;    butylated hydroxytoluene;    tBHP;    t-butylhydroperoxide;    DMNTA;    2;    2-dimethylnitrilotriacetase;    FCCP;    carbonyl cyanide-p-trifluoromethoxyphenylhydrazone;    PLA2;    phosphatidyl 2-acylhydrolase (EC 3.1.1.4);    Pi;    inorganic phosphate;    Propofol;    2;    6-di-isopropylphenol;    RLM;    rat liver mitochondria;    ΔΨ;    mitochondrial membrane potential;   

DOI  :  10.1016/0014-5793(91)80246-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molecular mechanisms of the Ca²⁺-induced permeabilization of rat liver mitochondria was evaluated by studying a new effect of the commonly used general anaesthetic Profol(2,6-diisopropylphenol). The compound was found to induce an apparent uptake of Ca²⁺ at steady-state in the Ca²⁺-distributed between the medium and the mitochondria, and to inhibit swelling and release of accumulated Ca²⁺ induced by inorganic phosphate, t-butyl hydroperoxide, diamide or FCCP plus Ruthenium red. The compound did not stimulate the activity of the Ca²⁺-uniporter and it is concluded that the effects seen are due to the inhibition of the Ca²⁺-dependent, unspecific permeability increase. The results suggest two mechanisms whereby Propofol stabilizes the mitochondrial membrane in the presence of Ca²⁺: (i) by interaction with the putative pore, thus causing its closure; and (ii) by scavenging of free radicals thus inhibiting its opening during oxidative stress.

【 授权许可】

Unknown