Human neurochordins: identification of several P‐epitope‐bearing polypeptides and a study of their ontogenetic expression

FEBS Letters

Rodionova, A.I.¹  Feshenko, S.P.²  Preobrazhensky, A.A.¹  Yaroshevich, E.Yu.² 

[1] A.N. Bach Institute of Biochemistry, USSR Academy of Sciences, Leninsky pr. 33, 117071 Moscow, USSR;Institute of Inherited and Congenital Diseases, BSSR Academy of Sciences, ul. Voroshilova 66, 220053 Minsk, USSR

关键词: High-M r glycoprotein;    P-epitope;    Nervous tissue;    Restricted tissue specificity;    Human embryo;   

DOI  :  10.1016/0014-5793(90)80799-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neurochordins are a family of immunologically related high-M _r neural tissue glycoproteins. After SDS-agarose electrophoresis of human neural tissue extracts, two main neurochordins (A1 and B2) as well as several minor ones (O, A2, A3, B1, B3, C1, C2, D) were vizualized on immunoblots stained with monoclonal antibody At5. Neurochordin expression starts in human embryos before 6 weeks of gestation. General antigenic activity of neurochordins increases between 6 and 24 weeks of gestation while its level does not alter from the second half of gestation up to the age of 11–13 years. Neurochordins extracted from large hemispheres of brain, from cerebellum and spinal cord of a 24-week embryo display a similar pattern after electrophoresis. Partially different pattern of neurochordins was observed with a brain tumor.

【 授权许可】

Unknown