Stimulation of actin synthesis in phalloidin‐treated cells

FEBS Letters

Serpinskaya, A.S.¹  Bershadsky, A.D.¹  Denisenko, O.N.¹  Gelfand, V.I.¹ 

[1] Institute of Protein Research, Academy of Sciences of the USSR, Pushchino, Moscow Region 142292, USSR

关键词: Scrape loading;    Phalloidin;    Actin synthesis;    Mouse fibroblast;    Feedback control;   

DOI  :  10.1016/0014-5793(90)80797-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We used the technique of scrape loading to introduce phalloidin into mouse embryo fibroblasts in mass culture. Phalloidin almost completely destroyed actin microfilament bundles, but the amount of polymerized cytoskeleton-associated actin was increased approximately two-fold and the amount of monomeric (Triton X-100 extractable) actin was significantly reduced. The major result of the present study is that the rate of actin synthesis in the phalloidin-treated cells was 2–3 times higher than in the control cells. Northern blot and translation in a cell-free system from rabbit reticulocytes showed that the actin mRNA level significantly increased as a result of phalloidin treatment.

【 授权许可】

Unknown