Dual effect of arachidonic acid on protein kinase C isoenzymes isolated from rabbit thymus cells

FEBS Letters

Buday, László¹  Faragó, Anna¹ 

[1] 1st Institute of Biochemistry, Semmelweis University Medical School, PO Box 260, Budapest 1444 8, Hungary

关键词: Protein kinase C isoenzyme;    Activation by cis-unsaturated fatty acid;    Arachidonic acid;    Thymus cell;   

DOI  :  10.1016/0014-5793(90)80547-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Type II and type III isoenzymes of protein kinase C isolated from rabbit thymus cells were activated at relatively low concentrations but were inhibited at higher concentrations of arachidonic acid. Activation by cis-unsaturated fatty acids required Ca²⁺ the maximal activity was approached at about 10⁻⁶ M Ca²⁺ concentration. The kinetics of activation and inhibition by arachidonic acid depended strongly on the nature of the substrate (synthetic oligopeptide or H1 histone), on the concentration of the protein substrate and on the stage of purification of the isoenzyme preparation investigated. Activation seemed to be favoured at high protein concentrations.

【 授权许可】

Unknown