FEBS Letters | |
Studies on the interaction of C1q,a subcomponent of the first component of complement, with porins from Salmonella minnesota incorporated into artificial membranes | |
Loos, Michael1  Latsch, Maria1  Möllerfeld, Jörg2  Ringsdorf, Helmut2  | |
[1] Institute of Medical Microbiology, Johannes Gutenberg-University, Augustusplatz/Hochhaus, D-6500 Mainz, FRG;Institute of Organic Chemistry, Johannes Gutenberg-University, Becherweg 18, D-6500 Mainz, FRG | |
关键词: C1q; Macrophage; Outer membrane protein; Liposome; Black lipid membrane; | |
DOI : 10.1016/0014-5793(90)80542-Q | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Purified outer membrane proteins (OMP) of Salmonella minnesota, Re-form, were incorporated into liposomes. These induced in macrophages a chemiluminescence signal identical to that of the intact Re-form. This signal was abolished by preincubation of porin-containing liposomes with purified C1q. Incorporation of isolated OMP into black lipid membranes (BLM) resulted in channel-formation which could not be inhibited by isolated C1q. Additionally, incubation of OMP-containing liposomes with BLM resulted in pore-formation within the BLM. This was amplified when lipid A was present within the liposomes. Preincubation of OMP-containing liposomes with purified C1q abolished pore-formation within the BLM.
【 授权许可】
Unknown
【 预 览 】
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RO201912020294283ZK.pdf | 450KB | download |