Evidence that the newly cloned low‐density‐lipoprotein receptor related protein (LRP) is the α2‐macroglobulin receptor

FEBS Letters

Kristensen, Torsten²  Moestrup, Søren K.¹  Sottrup-Jensen, Lars²  Gliemann, Jørgen¹  Bendtsen, Lone²  Sand, Ole² 

[1]Institute of Physiology, University of Aarhus, DK-8000, Århus C, Denmark

[2]Department of Molecular Biology, University of Aarhus, DK-8000, Århus C, Denmark

关键词: Low-density-lipoprotein receptor related protein;    α2-Macroglobulin;    α2-Macroglobulin receptor;    Placenta;    Liver;    Membrane protein structure;    LRP;    low density lipoprotein receptor related protein;    LDL;    low density lipoprotein;    VLDL;    very low density lipoprotein;    α2M;    α2-macroglobulin;    αM;    α-macroglobulin (collectively);    α2MR;    α2-maeroglobulin receptor;    SDS-PAGE;    sodium dodecylsulfate polyacrylamide gel electrophoresis;    HPLC;    high-performance liquid chromatography;    PVDF;    polyvinylidenedifluoride;   

DOI  :  10.1016/0014-5793(90)80530-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human placental receptor (α₂MR) for α₂-macroglobulin-proteinase complexes contains 3 polypeptides of approx. 500 kDa, 85 kDa, and 40 kDa. N-terminal sequence analysis of the 500 kDa and 85 kDa polypeptides, analysis of a random selection of peptides covering 536 residues from these polypeptides, and analysis of a 1772 bp cDNA encoding part of the 500 kDa polypeptide provide evidence that the 500 kDa and 85 kDa chains are the α- and β-subunits, respectively, of a recently cloned hepatic membrane protein, termed the low density lipoprotein receptor related protein (LRP) (Herz, J., Hamann, U., Rogne, S., Myklebost, O., Gausepohl, H. and Stanley, K.K. (1988) EMBO J. 7, 4119-4127; Herz, J., Kowal, R.C., Goldstein, J.L. and Brown, M.S. (1990) EMBO J. 9, 1769-1776). N-terminal sequence analysis of the 40 kDa polypeptide shows that it is of distinct genetic origin. It is suggested that LRP is the functional receptor for α₂-macroglobulin-proteinase complexes (α₂MR) and in addition may have as yet unsettled functions in lipoprotein metabolism.

【 授权许可】

Unknown