Refilling of endothelial calcium stores without bypassing the cytosol

FEBS Letters

Lückhoff, Andreas¹  Busse, Rudi¹ 

[1] Institute of Applied Physiology, University of Freiburg, Hermann-Herder-Straße 7, D-7800 Freiburg i.Br., FRG

关键词: Endothelial cell;    Intracellular Ca2+ store;    Calcium uptake inhibition;    2;    5-Di(tert-butyl)-1;    4-benzohydroquinone;    [Ca2+]c;    cytosolic free calcium concentration;    [Ca2+]e;    extracellular Ca2+;    BuBHQ;    2;    5-di(tert-butyl)-1;    4-benzo-hydroquinone;    InsP3;    inositol 1;    4;    5-trisphosphate;    InsP4;    inositol 1;    3;    4;    5-tetrakisphosphate;   

DOI  :  10.1016/0014-5793(90)80519-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present study was undertaken to define the route of Ca²⁺ used for refilling of intracellular Ca²⁺ stores in endothelial cells. Ca²⁺ stores, after emptying with bradykinin in Ca²⁺ free solution and termination of the stimulation with the bradykinin antagonist, Hoe 140, were allowed to refill by addition of Ca²⁺. Refilling was prevented by 2,5-di(tert-butyl)-1,4-benzohydroquinone (BuBHQ), an inhibitor of microsomal Ca²⁺ sequestration. BuBHQ induced large increases in the cytosolic Ca²⁺ concentration during the refilling phase. This finding is not compatible with a model proposing Ca²⁺ uptake into the stores directly from the extracellular space but provides evidence for uptake from the cytosolic compartment.

【 授权许可】

Unknown