Reversible ADP‐ribosylation of the 78 kDa glucose‐regulated protein

FEBS Letters

Ledford, Barry E.²  Leno, Gregory H.¹ 

[1] Molecular and Cellular Biology and Pathobiology Program, Medical University of South Carolina, Charleston, SC 29425 USA;Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425 USA

关键词: ADP-ribosylation;    GRP78/BiP;    Tunicamycin;    Amino acid starvation;    Nutritional stress;   

DOI  :  10.1016/0014-5793(90)80499-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Starvation of Mouse hepatoma cells for essential amino acids or glucose results in the mono-ADP-ribosylation of the 78 kDa glucose-regulated protein, GRP78. Here we show that the ADP-ribosylated and non-ADP-ribosylated forms of GRP78 are interconvertible during tryptophan starvation and refeeding. In addition, the ADP-ribosylation of GRP78 was shown to be reversible even during nutritional stress. The overexpressed pool of non-ADP-ribosylated GRP78 synthesized during tunicamycin treatment was available for ADP-ribosylation during subsequent amino acid starvation, especially in the absence of tunicamycin. The reversible ADP-ribosylation of GRP78 could be part of a metabolic control mechanism in operation during nutritional stress.

【 授权许可】

Unknown