Recognition of target colloid species by conjugates of a linear polyelectrolyte with a vector protein

FEBS Letters

Polynsky, A.S.¹  Chechik, O.S.¹  Yaroslavov, A.A.¹  Kabanov, V.A.¹  Sukhishvili, S.A.¹ 

[1] Department of Polymer Science, Faculty of Chemistry, Moscow State University, 119899, Moscow, USSR

关键词: Latex;    Polyelectrolyte adsorbtion;    Macromolecular interchange;    Enzyme-polycation conjugate;    Recognition phenomenon;    CPS-latex;    carboxylated polystyrene latex;    PCEMA-latex;    polychloromethylmetacrylate latex;    BSA;    bovine serum albumin;    TIS;    trypsin inhibitor from soybean;    CT;    α-chymotrypsin;    BTNPE;    N-benzyloxycarbonyl-L-tyrozine-p-nitrophenyl ester;    PEVP;    (4-venylpyridine/N-ethyl-4-vinylpyridinium bromide) copolymer (93:7);    PEAVP;    (4-vinylpyridine/N-4-vinylpyridinium bromide/N-carboxymethyl-4-vinylpyrydinium bromide) ternary coplymer (15:65:20);    CT-PEAVP;    conjugate of CT with PEAVP;    EPM;    electrophoretic mobility;   

DOI  :  10.1016/0014-5793(90)81478-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Redistribution reaction of quatermized poly-4-vinylpyridine polycations and their conjugates with x-chymotrypsin by oppositely charged latex particles is disclosed. The polycatrons are strongly absorbed on the latex surface. Nevertheless, they are able to migrate between the latex species via occasional interparticle contracts. In the case of a homogeneous latex such interchange results in uniform distribution of polycations by latex particles. The distribution drastically changes, when x-chymotrypsin-polycation conjugates interact with a mixture of two latexes; one chemically modified by bovine serum albumin and the other one by specific protein inhibitor of x-chymotrypsin. In this case the interchanging polycations are finally fixed on the latex particles carrying the centres of specific binding of the enzyme vector, i.e. recognize them in the latex mixture. The obtained results are considered to minus physico-chemical interaction and recognition of target supermolecular bio-objects by large macromolecules carrying relatively small molecular vectors.

【 授权许可】

Unknown