Detoxification of the macrolide toxin brefeldin A by Bacillus subtilis

FEBS Letters

Klöppel, Klauss-Dieter²  Kneusel, Richard E.¹  Matern, Ulrich¹  Wray, Victor² 

[1] Lehrstuhl für Biochemie der Pflanzen, Biologisches Institut II, Universität Freiburg, Schänzlestr 1, D-7800 Freiburg FRG;Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1,330 Braunschweig FRG

关键词: Brefeldin A detoxification;    Brefeldin A acid;    Brefeldin A lactonase;    Macrolide antibiotic;    Macrolide phytotoxin;    BFA;    brefeldin A;    BFA acid;    brefeldin A acid;    BMM;    bacterial minimal medium;    LB;    Luria-Bertani medium;   

DOI  :  10.1016/0014-5793(90)81450-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The macrolide brefeldin toxin A is a determinant of Alternaria leaf blight disease in safflower, which causes severe economic losses worldwide. Soilborne bacteria, classified as Bacillus subtilis spp., were isolated and shown to readily metabolize brefeldin A in laboratory culture to one major product. This product was identified by high resolution 2D ¹H NMR and FAB mass spectroscopies as the acid resulting from hydrolysis of the macrolide ring in brefeldin A. In contrast to brefeldin A, the acid completely lacked phytotoxic activity in the standard leaf bioassay. Detoxification of brefeldin A by the lactonase activity from Bacillus subtilis may be exploited in the future to introduce resistance to Alternaria leaf blight in sufflower.

【 授权许可】

Unknown