Construction and characterization of a recombinant tripartite enzyme, galactose dehydrogenase/β‐galactosidase/galactokinase

FEBS Letters

Ljungcrantz, Peter¹  Mosbach, Klaus¹  Bülow, Leif¹ 

[1] Pure and Applied Biochemistry, Chemical Center, P.O.B. 124, S-221 00 Lund, Sweden

关键词: Gene fusion;    Galactose dehydrogenase;    β-galactosidase;    Galactokinase;   

DOI  :  10.1016/0014-5793(90)81446-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The in-frame gene fusion between 3 enzymes, galactose dehydrogenase, β-galactosidase and galactokinase, is described. The purified artificial tripartite enzyme displayed all three enzymic activities. Two major forms of the hybrid protein were found, consisting of 4 and 8 subunits respectively, but other forms could also be identified. Each subunit was made up of one monomer each of galactose dehydrogenase, β-galactosidase and galactokinase. Proximity effects exhibited by the hybrid enzyme could be demonstrated using [¹⁴ C]galactose as a reporter molecule.

【 授权许可】

Unknown