Reversible thermal unfolding of Bacillus subtilis levansucrase is modulated by Fe3+ and Ca2+

FEBS Letters

Petit-Glatron, Marie-Françoise¹  Chambert, Régis¹ 

[1] Institut Jacques Monod, CNRS, Université Paris VII, Tour 43-2, place Jussieu, 75251 Paris Cedex, France

关键词: Thermal unfolding;    Protein secretion;    Levansucrase;    Bacillus subtilis;   

DOI  :  10.1016/0014-5793(90)81439-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The equilibrium transition curves for thermal unfolding of levansucrase were established at several pH values. At pH 7 and within the temperature range of bacterial growth, the unfolded from is predominant. However, under such conditions, refolding is promoted by the only addition of Ca²⁺ or Fe³⁺. We propose that the tertiary structure flexibility of levansucrase plays a key role in its secretion process.

【 授权许可】

Unknown