Is cytochrome P‐450scc a transmembrane protein?

FEBS Letters

Chashchin, Vadim L.¹  Chernogolov, Alexey A.¹  Usanov, Sergey A.¹ 

[1] Protein Chemistry Laboratory, Institute of Bioorganic Chemistry, BSSR Academy of Sciences, Minsk, USSR

关键词: Cytochrome P-450scc;    Fragment;    Monospecific antibody;    Membrane topology;    Bovine adrenal cortex;    P-450scc and P-45011β;    bovine adrenocortical cytochromes P-450scc and P-45011b;    F1;    F2 and F3;    fragments of P-450scc polypeptide chain;    PB;    sodium phosphate buffer;   

DOI  :  10.1016/0014-5793(90)81432-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The topology of cytochrome P-450_scc in the inner mitochondrial membrane of adrenal cortex has been investigated using monospecific antibodies to cytochrome P-450_scc and its fragments F₁ (Ile¹-Arg²⁵⁰), F₂ (Asn²⁵⁷-Ala⁴⁸¹) and F₃ (Asn²⁵⁷-Arg³⁹⁹). Antibodies to F₁ and F₂ were shown to effectively bind to the matrix and cytosolic sides of the inner membrane. Antibodies to F₃ specifically interacted only with the matrix side of the membrane. These data are consistent with a model of molecular organization which shows that cytochrome P-450_scc is a transmembrane protein, both N- and C-terminal sequences of the cytochrome being able to span the membrane.

【 授权许可】

Unknown