D1‐D2 complex of the photosystem II reaction center from spinach Isolation and partial characterization

FEBS Letters

Satoh, Kimiyuki¹  Tang, Xiao-Song¹  Fushimi, Kazuo¹ 

[1] Department of Biology, Faculty of Science, Okayama University, Okayama 700, Japan

关键词: Charge separation;    Cytochrome b-559;    D1-D2 complex;    Octyl-β-D-glucopyranoside;    Photosystem II reaction center;   

DOI  :  10.1016/0014-5793(90)81098-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A pigment-protein complex consisting of D1 and D2 proteins, but depleted in the two lower molecular mass components of photosystem II, i.e. cytochrome b-559 and psbI gene product, has been isolated by octyl-β-D-glucopyranoside treatment of the purified photosystem II reaction center complex from spinach [(1987) Proc. Natl. Acad. Sci. USA 84, 109-112], followed by separation by high performance liquid chromatography using a gel-permeation column (TSK G3000 SW). The isolated complex is photochemically active in the photoreduction of intrinsic pheophytin a under steady-state illumination, in the presence of dithionite and methyl viologen, and exhibits pigment stoichiometries similar to those in the untreated reaction center, indicating that the D1-D2 complex provides the site of primary photochemistry in photosystem II, as well as the principal binding sites of pigments in the reaction center.

【 授权许可】

Unknown