FEBS Letters | |
D1‐D2 complex of the photosystem II reaction center from spinach Isolation and partial characterization | |
Satoh, Kimiyuki1  Tang, Xiao-Song1  Fushimi, Kazuo1  | |
[1] Department of Biology, Faculty of Science, Okayama University, Okayama 700, Japan | |
关键词: Charge separation; Cytochrome b-559; D1-D2 complex; Octyl-β-D-glucopyranoside; Photosystem II reaction center; | |
DOI : 10.1016/0014-5793(90)81098-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A pigment-protein complex consisting of D1 and D2 proteins, but depleted in the two lower molecular mass components of photosystem II, i.e. cytochrome b-559 and psbI gene product, has been isolated by octyl-β-D-glucopyranoside treatment of the purified photosystem II reaction center complex from spinach [(1987) Proc. Natl. Acad. Sci. USA 84, 109-112], followed by separation by high performance liquid chromatography using a gel-permeation column (TSK G3000 SW). The isolated complex is photochemically active in the photoreduction of intrinsic pheophytin a under steady-state illumination, in the presence of dithionite and methyl viologen, and exhibits pigment stoichiometries similar to those in the untreated reaction center, indicating that the D1-D2 complex provides the site of primary photochemistry in photosystem II, as well as the principal binding sites of pigments in the reaction center.
【 授权许可】
Unknown
【 预 览 】
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