Long term culture of rat soleus muscle in vitro

FEBS Letters

Randle, Philip J.¹  Stace, Pamela B.¹  Marchington, David R.¹  Kerbey, Alan L.¹ 

[1] Nuffield Department of Clinical Biochemistry, John Radcliffe Hospital, Oxford OX3 9DU, UK

关键词: Muscle culture;    Muscle viability;    Glucose utilization;    Insulin sensitivity;    Adenosine;    Denervation;   

DOI  :  10.1016/0014-5793(90)81058-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat soleus muscle strips cultured for 24 h in medium 199 were well preserved in terms of electron microscopy; ATP and creatine phosphate concentrations; rates of glucose utilization, glycogen and protein synthesis, and effects of insulin thereon. Culture led to modest changes in fluid spaces and intracellular (K⁺); increased basal glucose utilization up to two-fold; had no effect on the maximum response to insulin; and had no effect on sensitivity to insulin except in the presence of adenosine deaminase. Thus in vitro neither denervation nor absence of insulin had any marked effects in 24 h to decrease responses to insulin.

【 授权许可】

Unknown