Signal transduction in human monocytes and granulocytes through the PI‐linked antigen CD14

FEBS Letters

Lund-Johansen, Fridtjof²  Bjerknes, Robert³  Olweus, Johanna²  Aarli, Ånen¹ 

[1] The Broegelmann Research Laboratory for Microbiology, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway;Department of Pathology, The Gade Institute, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway;Department of Pediatrics, University of Bergen, Haukeland Hospital, N-5021 Bergen, Norway

关键词: CD 14;    Signal transduction;    Calcium;    Oxidative metabolism;    Leukocyte;    Flow cytometry;   

DOI  :  10.1016/0014-5793(90)81049-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A possible role for the Pi-linked CD14 molecule in human monocyte and granulocyte signal mediation was investigated. Using flow cytometry and the fluorescent indicators Fluo-3 and dihydrorhodamine-123 it was shown that crosslinking of the CD14 molecule induces an increase in monocyte and granulocyte cytoplasmic calcium concentration and monocyte H₂O₂ production. These responses were found to be independent of IgG Fc receptors and suggest an intrinsic signal mediating capacity of the CD 14 molecule.

【 授权许可】

Unknown