Luminol enhanced chemiluminescence of the perfused rat heart during ischemia and reperfusion

FEBS Letters

Ikai, I.¹  Chance, B.¹  Kumar, C.¹  Okuda, M.¹ 

[1] Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6089, USA

关键词: Ischemia;    Reperfusion;    Heart;    Chemiluminescence;    Luminol;    LEC;    Luminol enhanced chemiluminescence;    EPR;    electron paramagnetic resonance;    LVDP;    left ventricular diastolic pressure;    LVSP;    left ventricular systolic pressure;    CF;    coronary flow;    KH buffer;    Krebs-Henseleit buffer;    KHL buffer;    Krebs-Henseleit buffer containing Luminol;    SOD;    Superoxide dismutase;    CAT;    catalase;   

DOI  :  10.1016/0014-5793(90)80463-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show that the production of Luminol reactive oxygen radicals in the perfused rat heart under ischemia and reperfusion can be monitored continuously by measuring the chemiluminescence of Luminol-perfused hearts. Luminol did not affect the monitored physiological parameters of the hearts. Chemiluminescence increased during ischemia and reperfusion. Superoxide dismutase treatment of the heart before ischemia, but not catalase, abolished these increases.

【 授权许可】

Unknown