Protein TAU variants present in paired helical filaments (PHFs) of alzheimer brains

FEBS Letters

Nunez, J.¹  Theiss, G.²  Guilleminot, J.¹  Ricolfi, F.¹  Gache, Y.¹ 

[1]INSERM U282-CNRS, Hôpital Henri Mondor, 94010 Créteil, France

[2]INSERM U282-CNRS, Bayer AG, Institut für Biochemie, 56000 Wuppertal 1, FRG

关键词: Alzheimer disease;    Paired helical filament;    Protein TAU;    NFTs;    neurofibrillary tangles;    PHFs;    paired helical filaments;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(90)80450-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyclonal anti-TAU antisera directed against native Tau protein and the NH₂-terminal side of the mouse TAU sequence were used to determine the nature of the TAU variants present in Alzheimer brains and in PHFs. These antibodies labelled specifically neuroflbrillary tangles and plaque neuntes in Alzheimer brains. On immunoblots of PHF extracts, two entities of 69 and 130 kDa were identified. These TAU-related species were absent from control brains. Protein immunoblot of total Alzheimer and control supernatants were shown to contain the same 4–5 TAU variants but none of the 69 and 130 TAU-related entities found in PHFs. These data suggest that specific TAU species are present in PHFs.

【 授权许可】

Unknown