Identification and structural analysis of a ribosomal RNA gene promoter from Thiobacillus ferrooxidans

FEBS Letters

Orellana, Omar¹  Jedlicki, Eugenia¹  Salazar, Oriana¹  Vargas, Danilo¹  Takamiya, Monica¹ 

[1] Departamento de Bioquimica, Facultad de Medicina, Universidad de Chile, Casilla 70086, Santiago 7, Chile

关键词: Acidophilic bacterium;    Ribosomal RNA;    Promoter;   

DOI  :  10.1016/0014-5793(90)80446-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 5'-terminus of a rRNA operon (rrnT₂) from Thiobacillus ferrooxidans was characterized. The rRNA promoters from this microorganism were identified by means of a functional assay in Escherichia coli. DNA sequencing of the promoter region, upstream the 16 S rRNA gene, showed the presence of a consensus sequence for bacterial ribosomal promoters. Other features such as a ‘discriminator’ sequence, antiterminator elements and an upstream hexanucleotide common to several rRNA operons were also found. Two other putative transcription promoters were also identified.

【 授权许可】

Unknown