Expression of subunit III ot the ATP synthase from spinach chloroplasts in Escherichia coli

FEBS Letters

Deckers-Hebestreit, Gabriele¹  Altendorf, Karlheinz¹  Burkovski, Andreas¹ 

[1] Universität Osnabrück, Fachbereich Biologie/Chemie, Arbeitsgruppe Mikrobiologie, Postfach 4469, D-4500 Osnabrück, FRG

关键词: F1Fo;    ATP synthase;    Fo complex;    Proteolipid;    Complementation;    DCCD;    N.N'-dicyclohexylcarbodiimide;    SDS;    sodium dodecyl sulfate;    IPTG;    isopropyl β-D-thiogalactopyranoside;   

DOI  :  10.1016/0014-5793(90)80412-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of subunit III of the ATP synthase from spinach chloroplasts in Escherichia coli has been achieved. Although the protein is inserted into the bacterial cytoplasmic membrane, formation of a functional F_o complex was not observed.

【 授权许可】

Unknown