Rearrangement and overexpression of the gene coding for tumor antigen p53 in a rat histiocytoma AK‐5

FEBS Letters

Khar, Ashok¹  Deshpande, Girish¹  Kumari, A.Leela¹ 

[1] Centre for Cellular and Molecular Biology, Hyderabad 500007, India

关键词: Rearrangement;    Overexpression;    p53;    Macrophage-like cell line;   

DOI  :  10.1016/0014-5793(90)80405-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene coding for the cellular tumor antigen p53 is rearranged and overexpressed in a rat histiocytoma, AK-5. The protein coded by the gene was detected by immunofluorescence and its full size was confirmed by immunoprecipitation using monoclonal antibodies against p53. Southern hybridizations with a full length cDNA probe specific for p53 indicated rearrangement of the gene. Alterations in the upstream region, which probably disrupt the normal regulatory control are suggested by the pattern obtained using a 5'-specific p53 probe in Southern hybridization.

【 授权许可】

Unknown