Inactivation of vesicular stomatitis virus by photosensitization following incubation with a pyrene‐fatty acid

FEBS Letters

Gatt, S.¹  Kotler, M.³  Fibach, E.²  Rabia, S.Abou¹ 

[1] Departments of Membrane Biochemistry and Newochemistry, Hebrew University N Hadassah School of Medicine, Jerusalem, Israel;Departments of Hematology, Hebrew University N Hadassah School of Medicine, Jerusalem, Israel;Departments of Molecular Genetics, Hebrew University N Hadassah School of Medicine, Jerusalem, Israel

关键词: VSV;    vesicular stomatitis virus;    P12;    12-(1-pyrene)-dodecanoic acid;    PFU;    plaque forming unit;    UVA;    long wavelength ultraviolet light;   

DOI  :  10.1016/0014-5793(90)81222-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vesicular stomatitis virus (VSV) was incubated with pyrene dodecanoic acid (P12), a fluorescent derivative of a medium-chain length fatty acid, and subjected to irradiation with a long wavelength ultra-violet light source at 366 nm (UVA). This inactivated the virus, resulting in a drastic decrease of its infectivity. The virus inactivation was proportional to the concentration of the pyrene-fatty acid, the length of exposure of the virus to P12 and the irradiation dose.

【 授权许可】

Unknown