Carnitine palmitoyltransferase (CPT2) from liver mitochondrial inner membrane becomes inhibitable by malonyl‐CoA if reconstituted with outer membrane malonyl‐CoA binding protein

FEBS Letters

Saggerson, E.David¹  Ghadiminejad, Iraj¹ 

[1] Department of Biochemistry, University College London, Gower Street, London WC1E 6BT, UK

关键词: Carnitine palmitoyltransferase;    Malonyl-CoA;    Liver;    Mitochondria;   

DOI  :  10.1016/0014-5793(90)81204-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A soluble extract was obtained on treatment of rat liver mitochondrial outer membranes with cholate which bound (¹⁴C]malonyl-CoA but was essentially free of carnitine palmitoyltransferase (CPT) activity. Extraction of mitochondrial inner membranes with cholate readily solubilized a CPT activity which was insensitive to malonyl-CoA. Combination of these two extracts caused the CPT derived from inner membranes to become inhibitable by malonyl-CoA.

【 授权许可】

Unknown