Immunochemical detection of photoaffinity‐labelled capsaicin‐binding proteins from sensory neurons

FEBS Letters

Walpole, Christopher¹  Wood, John N.¹  Dray, Andy¹  Coote, Paul R.¹  James, Iain F.¹ 

[1] The Sandoz Institute for Medical Research, 5 Gower Place, London WC1E 6BN, UK

关键词: Capsaicin;    Sensory neuron;    Receptor;    Photo-affinity labelling;   

DOI  :  10.1016/0014-5793(90)81198-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Capsaicin is a plant neurotoxin which depolarises a subset of mammalian sensory neurons. A photoaffinity probe (4-azidophenylpropionamide) with capsaicin-like agonist activity (EC₅₀ 5 μM) has been used to covalently label rat and chick sensory neurons in culture, as well as membrane preparations from both neurons and other tissues. Dorsal root ganglion cell specific capsaicin-binding proteins, including a major band of apparent molecular mass 58 000, have been identified by means of Western blotting, using a specific anti-capsaicin antiserum characterised by radioimmunoassay with a large range of capsaicin congeners. Using the same radioimmunoassay, no endogenous capsaicin-like immunoreactive material in normal or inflamed tissue has, however, been detected.

【 授权许可】

Unknown