FEBS Letters | |
Production and purification of a granular‐starch‐binding domain of glucoamylase 1 from Aspergillus niger | |
Belshaw, Nigel J.1  Williamson, Gary1  | |
[1] AFRC Institute of Food Research, Colney Lane, Norwich, Norfolk, NR4 7VA, UK | |
关键词: Glucoamylase; Binding domain; Starch binding; Proteolysis; Aspergillus niger; Gl; glucoamylase 1; G2; glucoamylase 2; G1C; C-terminal granular-starch-binding domain from glucoamylase 1 (residues 471–616); | |
DOI : 10.1016/0014-5793(90)81191-P | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A domain of glucoamylase 1 from Aspergillus niger which binds to granular starch was produced by proteolytic digestion and purified to apparent homogeneity by extraction with corn starch followed by anion-exchange chromatography and gel filtration. The peptide has a molecular weight of 25100, contains approximately 38% carbohydrate () and corresponds to residues 471–616 at the C-terminus of glucoamylase 1. The peptide bound to granular corn starch maximally at 1.08 starch. It inhibited the hydrolysis of granular starch by glucoamylase 1 but had no effect on the hydrolysis of starch in solution.
【 授权许可】
Unknown
【 预 览 】
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