Resonance Raman spectra of large pea phytochrome at ambient temperature

FEBS Letters

Tokutomi, Satoru²  Anni, Helen¹  Mizutani, Yasuhisa¹  Kitagawa, Teizo¹ 

[1] Institute for Molecular Science and Graduate University for Advanced Studies, Okazaki National Research Institutes, Myodaiji, Okazaki, 444 Japan;National Institute for Basic Biology, Okazaki National Research Institutes, Myodaiji, Okazaki, 444 Japan

关键词: Phytochrome;    Resonance Raman;    Vibrational spectra;    Protonation state of photochrome;   

DOI  :  10.1016/0014-5793(90)81189-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Resonance Raman (RR) scattering from large pea phytochrome was observed at ambient temperature for the first time by using a micro-spinning cell and the two color excitation technique. The relative population of the red-absorbing form (P_r), the far red-absorbing form (P_fr), and the bleached intermediate (I_bl) under laser illumination was estimated from the absorption spectra. The RR spectrum of P_r obtained by the 363.8 nm excitation under the 740.0 nm pumping exhibited a frequency shift between the H₂O and D₂O solutions, but those of P_fr and I_bl obtained by the 406.7 nm excitation under the 632.8 nm pumping did not, indicating a distinct difference in their protonation levels, presumably in the protonation of ring C.

【 授权许可】

Unknown