Comparison of the mode of action of ras p21 with those of protein kinases A and C in the stimulation of gene expression in NIH/3T3 cells

FEBS Letters

Fukumoto, Yasuo¹  Oku, Naohisa¹  Takai, Yoshimi¹  Fujioka, Hiroyuki¹  Hori, Yuichi¹  Kaibuchi, Kozo¹ 

[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan

关键词: ras p21;    Protein kinases A and C;    Enhancer/promoter;    NIH/3T3 cell;    SRE;    serum-response element;    CRE;    cyclic AMP-response element;    TRE;    12-O-tetradecanoylphorbol-13-acetateresponse element;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    protein kinase A;    cyclic AMP-dependent protein kinase;    SV40;    simian virus 40;    RS;    Rous sarcoma;    CAT;    chloramphenicol acetyltransferase;    cDNA;    complementary DNA;    Bt2cAMP;    dibutyryl cyclic AMP;    PDBu;    phorbol-12;    13-dibutyrate;    PDGF;    plateletderived growth factor;    NGF;    nerve growth factor;   

DOI  :  10.1016/0014-5793(90)81141-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To compare the mode of action of ras p21 with those of protein kinases A and C in the regulation of gene expression in NIH/3T3 cells, we investigated the transcriptional activity of various enhancer/promoters and enhancer motifs in the cells transfected with the c-Ha-ras ^val12 complementary DNA (cDNA). The results indicate that the c-Ha-ras ^val12 protein stimulates the enhancer/promoters of the c-fos gene, the metallothionein II_A gene, the simian virus 40 (SV40) virus genome and the Rous sarcoma (RS) virus genome, and the serum-response element and the 12-O-tetradecanoylphorbol-13-acetate (TPA)-response element in a manner independent of protein kinases A and C in NIH/3T3 cells.

【 授权许可】

Unknown