Mutual dependence of Na,K‐ATPase α‐ and β‐subunits for correct posttranslational processing and intracellular transport

FEBS Letters

Ackermann, U.¹  Geering, K.¹ 

[1] Institut de Pharmacologie de l'Université, Bugnon 27, CH-1005 Lausanne, Switzerland

关键词: Na;    K-ATPase;    Protein biosynthesis;    Protein oligomerization;    Glycosylation processing;    Protein degradation;    Xenopus laevis;    Oocyte;   

DOI  :  10.1016/0014-5793(90)81130-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study, we have followed the fate of newly synthesized α- and β-subunits of Na,K-ATPase in Xenopus oocytes injected with α and/or β cRNA to examine whether assembly of the two subunits is needed for a correct folding and/or for intracellular transport of Na,K-ATPase. Our data indicate that (1) assembly of α- and β-subunits occurs at the level of the ER, (2) β-subunits are needed for the newly synthesized α-subunit to adopt a stable configuration and (3) α- and β-subunits mutually depend on each other to be transported out of the ER.

【 授权许可】

Unknown