SecE‐dependent overproduction of SecY in Escherichia coli

FEBS Letters

Matsuyama, Shin-ichi¹  Mizushima, Shoji¹  Akimaru, Jiro¹ 

[1] Institute of Applied Microbiology, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113, Japan

关键词: SecY protein;    SecE protein;    Protein secretion;    SecY/SecE interaction;    Escherichia coli;    IPTG;    isopropyl-β-D-thiogalactopyranoside;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/0014-5793(90)81128-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The secY and secE genes were individually cloned and placed under the control of the tac promoter on plasmids. Induction with isopropyl-β-D-thiogalactopyranoside resulted in the overproduction of SecE, but not that of SecY. The simultaneous induced expression of both genes in the same cells resulted in the overproduction of SecY together with that of SecE. SecY and SecE thus overproduced were localized in the cytoplasmic membrane as those expressed at the normal levels were. It is suggested that SecY and SecE interact with each other in the cytoplasmic membrane. The numbers of the SecY and SecE molecules per cell were estimated.

【 授权许可】

Unknown