The influence of oligonucleotide‐effector on the selectivity of sequence specific modification of 16 S rRNA

FEBS Letters

Karpova, G.G.¹  Zenkova, M.A.¹  Fedorova, O.S.¹  Levina, A.S.¹  Mamaev, S.V.¹ 

[1] Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of USSR Academy of Sciences, 630090 Novosibirsk, USSR

关键词: Sequence specific modification;    Selectivity;    Oligonucleotide-effector;    R;    reagent;    2';    3'-O-[4-N-methyl-N-(2-chloroethyl)amino]benzylidene derivative of d(pTTTGCTCCCC)rA;    Phn;    N-(2-hydroxyethyl)phenazinium;    PAAG;    polyacrylamide gel;   

DOI  :  10.1016/0014-5793(90)81110-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constant of cooperative binding of the reagent and the oligonucleotide-effector with 16 S rRNA was determined. The accuracy of modification was shown to double in the presence of 50 μM effector at 5 μM concentration of the reagent.

【 授权许可】

Unknown