N‐Furfurylformamide as a pseudo‐substrate for formylmethanofuran converting enzymes from methanogenic bacteria

FEBS Letters

Thauer, R.K.²  Börner, G.²  Breitung, J.²  Berkessel, A.¹  Karrasch, M.² 

[1] Institut für Organische Chemie, J.W. Goethe-Universität, Niederurseler Hang, D-6000 Frankfurt/Main 50, FRG;Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität Marburg, Karl-von-Frisch-Straße, D-3550 Marburg/Lahn, FRG

关键词: Methanofuran;    Formylmethanofuran;    Methanogenesis;    Methanosarcina barkeri;    Methanobacterium thermoautotrophicum;    Coenzyme specificity;   

DOI  :  10.1016/0014-5793(90)81022-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methanofuran involved in methanogenesis. The N-formyl derivative is an intermediate in the reduction of CO₂ to CH₄ and the disproportionation of methanol to CO₂ and CH₄. Formylmethanofuran dehydrogenase and formylmethanofuran:tetrahydromethanopterin formyltransferase are the enzymes catalyzing its conversions. We report here that the two enzymes from Methanosarcma barkeri and the formyltransferase from Methanobacterium thermoautotrophicum can also use N-furfurylfonnamide as a pseudo-substrate albeit with higher apparent K _m and lower apparent V _max values. N-Methylformamide, formamide, and formate were not converted indicating that the furfurylamine moiety of methanofuran is the minimum structure required for the correct binding of the coenzyme.

【 授权许可】

Unknown