Endothelin stimulates hypertrophy and contractility of neonatal rat cardiac myocytes in a serum‐free medium

FEBS Letters

Mitsui, Youji¹  Hoshi, Hiroyoshi²  Suzuki, Takahiko² 

[1] Cell Science and Technology Division, Fermentation Research Institute, Agency of Industrial Science and Technology, Higashi 1-1-3, Tsukuba Science City, Ibaraki 305, Japan;Bio-Science Laboratory. Inc., 5-34-5, Shironishi, Yamagata 990, Japan

关键词: Endothelin;    Rat cardiac myocyte;    Hypertrophy;    Contractility;    Protein kinase C;   

DOI  :  10.1016/0014-5793(90)80995-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of endothelin (ET) on rat cardiac myocytes cultured in a serum-free, defined medium was determined. ET stimulated cardiac myocyte hypertrophy in a dose-dependent manner as determined by the protein synthesis and cell surface area. Since the myocyte hypertrophy was abolished by H-7, a protein kinase C inhibitor, ET-receptor mediated protein kinase C activation may be involved in cardiac myocyte hypertrophy. At the same time, ET also stimulated myocyte contractility in this medium, and this stimulatory effect was inhibited by nicardipine. This result indicates that the influx of extracellular calcium ion is necessary for the stimulation of contractility induced by ET.

【 授权许可】

Unknown