Exacerbation of rat adjuvant arthritis by intradermal injection of purified mammalian 14‐kDa group II phospholipase A2

FEBS Letters

Nakamura, Hideo¹  Inoue, Keizo²  Murakami, Makoto²  Yokoyama, Yuichi¹  Mori, Hiroshi²  Kudo, Ichiro² 

[1]Research Laboratories, Dainippon Pharmaceutical Co. Ltd, Osaka 564, Japan

[2]Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Phospholipase A2;    Adjuvant arthritis;    Carrageenan-induced edema;    ELISA;    enzyme-linked immunosorbent assay;    EDTA;    ethylenediamine tetraacetate;    PBS;    phosphate-buffered saline;    SDA-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(90)80986-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Appreciable phospholipase A₂ activity was detected in a hind paw homogenate from rats with adjuvant arthritis or carrageenan-induced edema. The activity was neutralized by treatment with antibody raised against rat platelet secretory phospholipase A₂, indicating that 14-kDa group II phospholipase A₂, was induced in the hind paw during the process of inflammation. Injection of purified rat platelet phospholipase A₂ into the hind paw of rats with adjuvant-induced arthritis resulted in exacerbation of edema in a dose-dependent manner, whereas no effect was observed on either normal rats or animals with carrageenan-induced edema under the same experimental conditions. These results suggest the importance of mammalian group II phospholipase A₂, in the pathogenesis of some types of inflammation.

【 授权许可】

Unknown