The cholecystokinin gene is abundantly co‐expressed with gastrin‐releasing peptide, enkephalin and neuropeptide Y genes in a clonal human neuroepithelioma cell line

FEBS Letters

Burbach, J.Peter H.¹  Verbeeck, Marleen A.E.¹ 

[1] Rudolf Magnus Institute, Medical Faculty, University of Utrecht, Vondellaan 6, 3521 GD Utrecht, The Netherlands

关键词: Cholecystokinin;    Neuropeptide co-expression;    Neuronal cell line;    Gene expression;    Enkephalin;    Gastrin-releasing peptide;   

DOI  :  10.1016/0014-5793(90)80979-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cholinergic human neuroepithelioma cell line SK-N-MCIXC expressed mRNAs for the neuropeptides cholecystokinin (CCK), neuropeptide Y, gastrin-releasing peptide (GRP) and enkephalin. The CCK transcript of about 800 nt was present at very high levels and CCK-like peptides immunoreactive to a C-terminal CCK octapeptide antiserum were present in the cell line and its medium. This clonal neuronal cell line provides a unique model system to identify cis- and trans-acting factors responsible for neuron-specific expression and regulation of the CCK gene. Further-more, the pluripotent properties of the undifierentiated cell line may open studies on neuronal differentiation at the level of co-expression of neuropeptides and transmitters.

【 授权许可】

Unknown